Page finder 1. Health and Safety 1.1. Scope 1.2. Intended use 1.3. Safety Notices 1.4. General Safety 1.5. General Hazards 1.6. Unpacking and Installation 2. Operating and Maintenance 2.1. Preparation before starting 2.2. Performing a run - cuvettes 2.3. Performing a run – Micro Volume Instruments 2.4.
• Ultrospec 2100/7000/8000/9000 UV/Visible spectrophotometers • SimpliNano™ and NanoVue™ Plus micro volume spectrophotometer Translations of these instructions into 22 European languages are available on the User Manual CD supplied with the instrument and on the internet at GE home page www.gelifesciences.com 1.2. Intended use Visible and UV/Visible spectrophotometers shine light through a liquid sample and measure its Absorbance.
WARNING • There are no bio-hazardous materials within the unit; however this unit could be used with bio-hazardous samples. Before using the instrument, the customer should have in place decontamination procedures designed to protect laboratory workers from occupationally acquired infections. The sample chamber cell holders are removable and may be decontaminated using the appropriate disinfectant for the bio hazard in question, rinsed with distilled water and then allowed to dry.
The unit should not be operated with the sample chamber lid open or the lamp housing lid removed as prolonged exposure to the beam may cause permanent eye damage.(Not applicable to Ultrospec 10, Novaspec III/Plus) WARNING High voltages exist inside the Ultrospec 2100/7000/8000/9000, GeneQuant 100/1300, SimpliNano and NanoVue Plus instruments. Repair and maintenance should only be carried out by individuals trained specifically to work on these instruments.
Page 6
• The spectrophotometer may be fitted with a range of accessories for specific applications. For the GeneQuant 100/1300 and NanoVue Plus these include a built in printer, bluetooth or SD card accessory. The SimpliNano has an optional built- in printer. The Ultrospec 2100/7000/8000/9000 have a range of accessories that can be fitted in the sample compartment including a sipper, long path length cell holder and thermostatted cell holder.
All spectrophotometers are operated in the same generic way by comparing the amount of light Absorbed by a sample when compared to a reference solution: 2.1. Preparation before starting • The instrument is switched on and allowed to initialise, Ultrospec 10, Novaspec III/Plus, GeneQuant 100/1300, SimpliNano and NanoVue Plus units have the on/off switch on the keypad. • Ultrospec 2100 units have an on/off switch on the back of the instrument on the right hand side.
• Typical results screen (DNA application GeneQuant) 2.3. Performing a run - micro volume instruments • O n the SimpliNano units samples are measured in a sample port. Using a pipette a solution of reference material, which is typically the solution that the sample is dissolved in, is dropped onto the measurement position in the sample port and the...
SimpliNano – wipe the sample port with a dry, lint-free tissue between samples and at the end of the run, this is sufficient to remove sample. If more thorough cleaning is desired, the port can also be cleaned with dilute (2%) detergent solution, followed by isopropyl alcohol.
Cleaning of sample plates, recalibration of NanoVue Plus units pathlength SimpliNano units Cleaning of sample port 2.6. Novaspec III/Plus Lamp Change 1. Switch off the instrument, remove the sample from the cell holder and disconnect the power supply cord 2.
3. TROUBLESHOOTING Detailed in the table below are the most common faults found whilst using our spectrophotometers Instrument Problem Possible causes Ultrospec 10 A flashing Absorbance reading of 2.00 Abs is This indicates an Absorbance of more than obtained. 1.99 and is therefore out of range. The sample needs to be diluted.
3.3. Recycling of hazardous substances Spectrophotometers contain hazardous substances. Detailed information is available from your local GE representative. 3.4. Disposal of electrical components Waste of electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately.
Ultrospec 10, 2100Family Cambridge, September 2013 Ultrospec 7000, 8000, 9000 Family Cambridge, September 2013 SimpliNano Family Cambridge October 2013 Identity of person authorized to sign DoC Howie Pether, Managing Director Full specification and operating instructions are contained within the relevant user manual.
4. LEGAL GE, GE monogram and imagination at work are trademarks of General Electric Company. Nanovue and SimpliNano are trademarks of GE Healthcare Company or one of its subsidiaries. Ultrospec, Novaspec and GeneQuant are trademarks of Biochrom Ltd. All other third party trademarks are the property of their respective owner.
Page 15
Page intentionally left blank 29-0047-78 AC 04-2014...
GE Healthcare offices: GE Healthcare Bio-Sciences AB Björkgatan 30, 751 84 Uppsala, Sweden GE Healthcare Europe GmbH Munzinger Strasse 5, D-79111 Freiburg, Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, GE Healthcare Japan Corporation Sanken Bldg.