hit counter script
  1. Manuals
  2. Brands
  3. DAS Manuals
  4. Laboratory Equipment
  5. AP-BLOT
  6. Service manual

DAS AP-BLOT Service Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
SERVICE MANUAL
SERVICE MANUAL
SERVICE MANUAL
SERVICE MANUAL
This manual contains DAS reserved information. All rights reserved. Unauthorized copying of this manual or parts
is prohibited.
das
srl
AP- - - - BLOT
AP
BLOT
AP
AP
BLOT
BLOT
Viale Tivoli Km 18,642 – 00018 Palombara Sabina (Roma) - Italy
Tel. (+39) 0774 66840 – 0774 637070 - Fax 0774 634039
E - m a i l : i n f o @ d a s i t a l y . c o m - h t t p : / / w w w . d a s i t a l y . c o m

Advertisement

Table of Contents
loading

Summary of Contents for DAS AP-BLOT

  • Page 1 SERVICE MANUAL SERVICE MANUAL SERVICE MANUAL This manual contains DAS reserved information. All rights reserved. Unauthorized copying of this manual or parts is prohibited. Viale Tivoli Km 18,642 – 00018 Palombara Sabina (Roma) - Italy Tel. (+39) 0774 66840 – 0774 637070 - Fax 0774 634039...
  • Page 3 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 3 SECTION 0 Date: 02.04.2009 INTRODUCTION Index Date Description Execution Verification Approval Indice Data Descrizione Elaborazione Verifica Approvazione Issue Rev. 00 29.04.08 General revision Rev. 01 02.04.09 Important Notes: To avoid errors during maintenance and repairs, carefully read the entire manual before attempting any work on the instrument.

  • Page 5: Table Of Contents

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 5 SECTION 0 Date: 02.04.2009 INTRODUCTION CONTENTS SECTION 1 GENERAL SAFETY ..........................9 1.1. GENERAL SAFETY WARNINGS AND SYMBOLS................11 1.2. INSTRUMENT USE AND CONDITIONS....................11 1.3. INTENDED USE/USERS OF THE INSTRUMENT................. 11 SECTION 2 INSTALLATION AND TECHNICAL SPECIFICATIONS ............
  • Page 6 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 6 SECTION 0 Date: 02.04.2009 INTRODUCTION 3.5.4.3. Substituting Z Axis probe ..........................47 3.5.4.4. Substituting Z Axis probe motor........................48 3.6. SHAKING PLANE............................ 49 3.6.1. DESCRIPTION OF HOW IT WORKS ....................49 3.6.1.1. Shaking plane movement mechanism ......................49 3.6.1.2.
  • Page 7 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 7 SECTION 0 Date: 02.04.2009 INTRODUCTION 4.7.9. Exit ................................. 83 4.8. DILUTOR..............................84 4.8.1. Calibrate probe positions........................84 4.8.2. Calibrate probe height ........................... 89 4.8.3. Calibrate LT ............................91 4.8.4. Calibrate shaking plane ......................... 93 4.8.5.

  • Page 9: Section 1 General Safety

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 9 SECTION 1 Date: 02.04.2009 GENERAL SAFETY SECTION 1 GENERAL SAFETY...

  • Page 11: General Safety Warnings And Symbols

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 11 SECTION 1 Date: 02.04.2009 GENERAL SAFETY WARNINGS 1.1. GENERAL SAFETY WARNINGS AND SYMBOLS In this manual the following symbols stand for danger or warnings: General DANGER symbol which indicates that a serious safety risk can occur if instructions and warnings are not followed.

  • Page 13: Section 2 Installation And Technical Specifications

    Doc. N° MDS-AP-26-00-02 AP Blot – SERVICE MANUAL Rev.01 Pag. 13 SECTION 2 Date: 02.04.2009 INSTALLATION AND TECHNICAL SPECIFICATIONS SECTION 2 INSTALLATION AND TECHNICAL SPECIFICATIONS...

  • Page 15: Installation

    Doc. N° MDS-AP-26-00-02 AP Blot – SERVICE MANUAL Rev.01 Pag. 15 SECTION 2 Date: 02.04.2009 INSTALLATION AND TECHNICAL SPECIFICATIONS 2.1. INSTALLATION The instrument is packed with a wooden box. To unpack the instrument follow the instructions described below: Remove the 2 plastic covers (1-2) from the instrument right side and the other ones from the left side. Screw in the 4 handles on both sides (3-4) for manual instrument placement.

  • Page 16: Work Area

    AP Blot – SERVICE MANUAL Rev.01 Pag. 16 SECTION 2 Date: 02.04.2009 INSTALLATION AND TECHNICAL SPECIFICATIONS 2.2. WORK AREA AP-Blot work area showing the various components: Probe washing 48 position Sample rack Reagent and control rack Tanks basin Camera Calibration area Strip holder rack Fig.

  • Page 17: Section 3 Maintenance Procedures

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 17 MAINTENANCE PROCEDURES Date: 02.04.2009 SECTION 3 MAINTENANCE PROCEDURES...

  • Page 19: System Architecture

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 19 MAINTENANCE PROCEDURES Date: 02.04.2009 SYSTEM ARCHITECTURE The system architecture is illustrated in the following diagram and described in table below: Function group DILUTOR SHAKING PLANE UNIT X-Y AXIS Z1, Z2 AXIS CAMERA DILAP21...
  • Page 20 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 20 MAINTENANCE PROCEDURES Date: 02.04.2009 The AP Blot communicates with the PC via a serial connection: between the Dilutor PCB and the PC. The units controlled by the PCB are: Unit X –...

  • Page 21: Removing Work Area

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 21 MAINTENANCE PROCEDURES Date: 02.04.2009 REMOVING WORK AREA 3.2.1. REMOVING WORK AREA Turn off instrument To remove strip holder tray plate first loosen four screws holding shaking plane 4 fixing screws Remove 2 fixing screws from Probe wash basin and detach Waste (“OUT”) tube from under basin...
  • Page 22 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 22 MAINTENANCE PROCEDURES Date: 02.04.2009 Remove 8 screws from bottle separation carter Remove 7 screws from aluminium shaking plane Bottle separation carter Aluminium shaking plane Lift up and detach screws holding the shaking plane movement mechanism motor NB: each time shaking plane support is moved, or removed calibrate shaking plane as described Para.

  • Page 23: Removing Shaking Plane

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 23 MAINTENANCE PROCEDURES Date: 02.04.2009 3.2.2. Removing shaking plane Turn off device Remove 8 screws from strip holder tray plate. 8 fixing screws Remove strip holder tray plate strip holder tray plate Lift up shaking plane...

  • Page 24: Dilutor Unit

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 24 MAINTENANCE PROCEDURES Date: 02.04.2009 DILUTOR UNIT 3.3.1. DESCRIPTION OF HOW IT WORKS FUNCTION The dilutor unit aspirates and dispenses samples, calibrators, controls and all reagents, and also cleans inside of Z probe.

  • Page 25: Dilutor Mechanism

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 25 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.1.2. Dilutor mechanism This mechanism moves the syringe plunger (1) up and down in small steps. The movement is generated by a step motor (2) which moves a special worm screw (3) inside a female plastic nut 4) fixed on a carriage (5) which runs along a track (6) on 4 ball bearings.

  • Page 26: Pump Unit

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 26 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.1.4. Pump Unit Peristaltic pump A Peristaltic pump tubing Peristaltic pump B Peristaltic pump tubing The pump unit is made up of 2 peristaltic pumps composed of: rotating wheel stand peristaltic pump tubing...

  • Page 27: Probe

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 27 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.1.5. Probe The probe has two functions: Liquid aspiration and dispensation • Liquid sensor (for liquid level) • Aspiration and dispensation The probe aspirates and dispenses controls, samples, etc. Microprocessor Aspiration Probe...

  • Page 28: Dilutor Unit Pcb Electrical Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 28 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.1.7. Dilutor Unit PCB Electrical Diagram The table below describes each PCB and the various dilutor unit components they control and the diagram shows the PCBs and the dilutor unit electrical circuit.

  • Page 29: Dilutor Unit Hydraulic Component Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 29 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.1.8. Dilutor unit hydraulic component diagram The table below describes the different functions of the dilutor unit hydraulic components. The diagram shows how the dilutor unit circuit is set up to work. WASH bottle Contains probe-washing liquid, 5 litre capacity Level Sensor...

  • Page 30: Regular Dilutor Unit Maintenance

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 30 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.2. REGULAR DILUTOR UNIT MAINTENANCE Visually check the following components: Tubing connecting Valve – Probe. − Syringe plunger − Syringe − Every 4 months Ref.

  • Page 31: Substituting Dilutor Unit Components

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 31 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.3. SUBSTITUTING DILUTOR UNIT COMPONENTS To substitute syringe and syringe plunger refer to para. 4.8.5. The following table lists procedures and paragraphs relevant to these substitutions. Substituting valve –...

  • Page 32: Substituting Valve Mechanism

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 32 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.3.2. Substituting valve mechanism Turn on instrument. Run XCalib Program (See Section 4). From “Dilutor” pulldown menu select “Calibrate syringe” and follow instructions. Click on “Remove syringe”...

  • Page 33: Substituting Peristaltic Pump Tubing

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 33 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.3.5. Substituting Peristaltic Pump Tubing Turn on instrument. Run XCalib program. From “Dilutor” menu select “Prime” Detach wash loading and buffer tubing from their bottles Set prime for at least 60 seconds to empty hydraulic circuit.

  • Page 34: Substituting Dilutor Motor

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 34 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.3.6. Substituting Dilutor Motor Remove AWG15 tubing. Remove 4 screws from dilutor unit stand. Remove connector that joins dilutor motor to INTERFA2 PCB Loosen two grub screws that block motor axis mechanism .

  • Page 35: Substituting Opto Sidilop Pcb

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 35 MAINTENANCE PROCEDURES Date: 02.04.2009 3.3.3.7. Substituting Opto SIDILOP PCB Remove AWG15 tubing. Remove 4 screws from dilutor panel. Remove connector between SIDILOP PCB and INTERFA2 PCB. Unscrew fixing screws. Re-position new PCB and attach it with fixing screws Re-connect new SIDILOP and INTERFA2 PCB.

  • Page 36: X-Y Axis

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 36 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4. X–Y AXIS 3.4.1. DESCRIPTION OF HOW IT WORKS 3.4.1.1. X – Y axis movement mechanism FUNCTION: The X – Y axis movement mechanism moves probe arm precisely over work area. The X axis movement mechanism moves the X axis carriage (1).

  • Page 37: X- Y Axis Electrical Functions Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 37 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.1.2. X- Y axis Electrical Functions Diagram The table below describes the electrical components of each PCB which effects the movement of the X-Y axis. The arrangement is shown in the diagram.

  • Page 38: Regular Maintenance For X-Y Axis

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 38 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.2. REGULAR MAINTENANCE FOR X–Y AXIS Cleaning X-Y axis tracks Every 6 months Ref. para. 3.4.2.1 Check belt tension Every 6 months Ref. para. 3.4.2.2 Check condition of flat cables Every 6 months Ref.

  • Page 39: Substituting X-Y Axis Parts

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 39 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.4. Substituting X–Y AXIS PARTS Substituting X-axis Movement Motor Ref. para. 3.4.4.1 Substituting Y-axis Movement Probe motor Ref. para. 3.4.4.2 Substituting Opto XGOLAV (X-axis movement) Ref.

  • Page 40: Substituting Y-Axis Movement Probe Motor

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 40 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.4.2. Substituting Y-axis Movement Probe Motor Remove top cover by undoing screws on top of device. Remove connector which joins motor to AP22MOT PCB Remove motor after loosening 4 fixing screws.

  • Page 41: Substituting X-Axis Movement Belt

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 41 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.4.4. Substituting X-axis movement belt Remove screws fixing instrument cover to remove cover. Loosen 3 grub screws fixing both ends of belt. Replace belt and then tighten 3 grub screws which fixed the 2 ends of belt. Check that belt is correctly tightened.

  • Page 42: Substituting Interop2 Pcb (Y-Axis Movement)

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 42 MAINTENANCE PROCEDURES Date: 02.04.2009 3.4.4.7. Substituting INTEROP2 PCB (Y-axis movement) Undo fixing screws to remove device top cover Detach connectors from INTEROP2 PCB and remove 4 fixing screws. 2 fixing screws 2 fixing screws Reposition new PCB and secure it with 4 fixing screws.

  • Page 43: Z1 - Z2 Axis

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 43 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5. Z1 - Z2 AXIS 3.5.1. DESCRIPTION OF HOW IT WORKS 3.5.1.1. Z1 - Z2 axis movement mechanism FUNCTION OF Z AXIS (dispensation) Axis moves the dispensation probe up and down over the instrument work area.

  • Page 44: Z Axis Electrical Circuit Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 44 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5.1.2. Z axis electrical circuit diagram The diagram shows the different electronic functions with the relevant PCBs for the Z axis, followed by a brief description and the following table lists the various PCBs and their components.

  • Page 45: Regular Z Axis Maintenance

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 45 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5.2. REGULAR Z AXIS MAINTENANCE 3.5.2.1. axis movement control Turn on device and run XCalib programme. From “Dilutor” pulldown menu select “Calibrate height” then select a strip holder plate and select probe 1, Select “Go down”...

  • Page 46: Substituting Z And Zaxis Parts

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 46 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5.4. SUBSTITUTING Z and Z AXIS PARTS Substituting Z1 Axis probe Para. 3.5.4.1 Substituting Z1 Axis probe motor Para. 3.5.4.2 Substituting Z2 Axis probe Para.

  • Page 47: Substituting Z Axis Probe Motor

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 47 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5.4.2. Substituting Z Axis probe motor Remove instrument cover Remove Z axis protective carter by unscrewing two fixing screws Remove motor connector from INTEROP2 PCB and remove cable holder bands Motor fixing screws Remove motor fixing screws...

  • Page 48: Substituting Z 2 Axis Probe Motor

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 48 MAINTENANCE PROCEDURES Date: 02.04.2009 3.5.4.4. Substituting Z Axis probe motor Remove Z protective carter after undoing two fixing screws 2 fixing screws Remove motor connector from INTEROP2 PCB and remove cable holder bands Remove fixing screws and change motor Motor fixing screws...

  • Page 49: Shaking Plane

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 49 MAINTENANCE PROCEDURES Date: 02.04.2009 3.6. SHAKING PLANE 3.6.1. DESCRIPTION OF HOW IT WORKS 3.6.1.1. Shaking plane movement mechanism The shaking plane moves (shakes) the slides with the strips to be processed. The slides are placed on the ‘hinged’ shaking plane (1) and the movement is driven by a worm screw (2) on a ball-bearing (3) which moves along a wedge (4) mounted under the shaking plane.

  • Page 50: Shaking Plane Circuit Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 50 MAINTENANCE PROCEDURES Date: 02.04.2009 3.6.1.2. Shaking plane circuit diagram The table lists the PCBs and the electrical components that move the Shaking plane. This is followed by a diagram of the mechanism. APE-IF-DIL-AP21 PCB Z1 and Z2 axis motor drivers OP-SWING PCB...

  • Page 51: Maintenance And Shaking Plane Calibration

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 51 MAINTENANCE PROCEDURES Date: 02.04.2009 3.6.2. MAINTENANCE AND SHAKING PLANE CALIBRATION For calibration and maintenance of shaking plane refer to para. 4.8.4. The following table lists the calibration procedures described in the relevant paragraphs. 3.6.3.

  • Page 52: Substituting Opto Pcbs

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 52 MAINTENANCE PROCEDURES Date: 02.04.2009 3.6.3.2. Substituting opto PCBs Remove work area as described in Para. 3.2.1. Detach connector from Opto PCB to be substituted Remove fixing screws to undo PCB Reposition new PCB check that the Opto is not touching the encoder and replace the connector Reposition work area Calibrate shaking plane...

  • Page 53: Camera

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 53 MAINTENANCE PROCEDURES Date: 02.04.2009 3.7. CAMERA 3.7.1. DESCRIPTION OF HOW IT WORKS The camera is used to acquire digital images of each single strip. These images are then automatically elaborated by the software and each single blot is analysed.

  • Page 54: Camera Maintenance

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 54 MAINTENANCE PROCEDURES Date: 02.04.2009 3.7.2. CAMERA MAINTENANCE Check USB cable connection Para. 3.7.2.1 Lens cleaning Para. 3.7.2.2 3.7.2.1. Checking USB cable connection Check that USB cable is correctly connected to the camera. When cable is correctly connected then check that the camera is communicating with PC Turn on device.

  • Page 55: Substituting Camera Lens

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 55 MAINTENANCE PROCEDURES Date: 02.04.2009 3.7.4.2. Substituting camera lens Turn off device Manually move Z-axis unit out of the way to ensure free access to lens Detach USB cable from camera Remove 3 hexagonal head screws fixing camera to stand Unscrew lens in clockwise direction then remove it Screw in a new lens onto camera and block it to the stand with the 3 hexagonal head screws...

  • Page 56: Optical Sensors

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 56 MAINTENANCE PROCEDURES Date: 02.04.2009 3.8. OPTICAL SENSORS 3.8.1. DESCRIPTION OF HOW OPTICAL SENSORS FUNCTION The optical sensors detect the position or movement of a mechanism and translates this information into an electrical signal.

  • Page 57: Checking Distance For Opto Couplers (X Axis)

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 57 MAINTENANCE PROCEDURES Date: 02.04.2009 3.8.3. CHECKING DISTANCE FOR OPTO COUPLERS (X AXIS) Click on Tcom exe icon to open Tcom program Select DIL PCB. Type in HH to position probe in Home position. TypeMX5 Type XKX.

  • Page 58: Checking Distance For Opto Couplers (Y Axis)

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 58 MAINTENANCE PROCEDURES Date: 02.04.2009 3.8.4. CHECKING DISTANCE FOR OPTO COUPLERS (Y AXIS) Click on Tcom exe icon to open Tcom program Select DIL PCB. Type in HH to position probe in Home position. TypeMY5 Type XKY.

  • Page 59: Usb Communication

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 59 MAINTENANCE PROCEDURES Date: 02.04.2009 3.9. USB COMMUNICATION 3.9.1. DESCRIPTION OF HOW IT WORKS FUNCTION The instrument and PC can communicate through a USB connection. COMPOSITION DILAP21 Personal Computer 3.9.2.

  • Page 60: Replacing Dilap21 And Sir4Mot Pcbs

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 60 MAINTENANCE PROCEDURES Date: 02.04.2009 3.9.3. REPLACING DILAP21 and SIR4MOT PCBs To remove right back panel undo two fixing screws DIL-AP21 SIR4MOT2 Locate DILAP21 or SIR4MOT2 PCBs and remove all connectors Undo 4 fixing screws to remove PCB Replace PCB and reposition connectors Close right back panel and replace fixing screws...

  • Page 61: Dilutor Command List

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 61 MAINTENANCE PROCEDURES Date: 02.04.2009 3.10. DILUTOR COMMAND LIST Sets serial number (maximum 8 characters). The character ‘ ‘ is not considered. Closes TEST MODE and returns to command mode All characters followed by “;”...
  • Page 62 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 62 MAINTENANCE PROCEDURES Date: 02.04.2009 Takes X and Y axis to Home position. Takes X and Y axis to Home position, moving ZZ to slit 1 and Z1 and Z2 along steps defined by HA command.
  • Page 63 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 63 MAINTENANCE PROCEDURES Date: 02.04.2009 Turns off all motors Turns on waste pump Turns off waste pump Turns on aspiration pump, waste pump 2 and opens aspiration electro-valve on the wash basin Creates a logic signal of 100 msecs.
  • Page 64 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 64 MAINTENANCE PROCEDURES Date: 02.04.2009 clears timer 2 Memorises ‘c’ variable from a vector of 50 elements (1 ≤c ≤ 50). Average difference between timer 2 TC c,i,j vector elements ‘i’...
  • Page 65 AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 65 MAINTENANCE PROCEDURES Date: 02.04.2009 Quick Short-Cut Commands Are there any commands to execute? (answer: y / n) Exits from wait time (see time commands) Displays wash bottle status Displays buffer bottle status Ignores liquid error handling set by SEE command Shows last typed command...

  • Page 66: Hydraulic Circuit Diagram

    AP Blot - SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 3 Rev.01 Page 66 MAINTENANCE PROCEDURES Date: 02.04.2009 3.11. HYDRAULIC CIRCUIT DIAGRAM...

  • Page 67: Regular Maintenance

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 67 OPERATING PROCEDURES Date: 02.04.2009 3.11.1. REGULAR MAINTENANCE For correct maintenance of all hydraulic circuit parts refer to paras. 4.7.1. and 4.8.8. The next table shows the sections dealing with routine maintenance checks Prime Para.

  • Page 68: Summary Of Regular Maintenance

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 68 OPERATING PROCEDURES Date: 02.04.2009 3.12. SUMMARY OF REGULAR MAINTENANCE 3.12.1. DILUTOR UNIT Operation How often Reference Visual checks Every 4 months Para. 3.3.2.1 3.12.2. X–Y AXIS Operation How often Reference Cleaning X-Y axis tracks...

  • Page 69: Section 4 Calibration Procedures (Xcalib)

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 69 CALIBRATION PROCEDURES (XCalib) Date: 02.04.2009 SECTION 4 CALIBRATION PROCEDURES (XCALIB)

  • Page 71: Installing Software

    SECTION 4 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 XCALIB is the service software to set up and check all AP-Blot parameters. This software is complementary to the main AP-Blot management software. 4.1. INSTALLING SOFTWARE Close down all applications and insert “APEX software” CD in PC. The APEX software is self-installing. If the installation procedure does not start up automatically then launch it by clicking the setup.exe folder which is displayed...

  • Page 72: Operating Menu

    Doc. N° MDS-AP-26-00-02 AP Blot– SERVICE MANUAL Rev.01 Pag. 72 SECTION 4 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.2. OPERATING MENU This table shows how the software is set up and the different functions. Main function Dilutor Settings Calibrate probe position Tank level sensors Language Instrument information...

  • Page 73: Description Of Menu Functions

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 73 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.3. DESCRIPTION OF MENU FUNCTIONS Menu Description Main functions Tank level sensors Checks tank level sensors Dispensation Test Only enabled for APE set up Dilution Test Only enabled for APE set up Movement Test...

  • Page 74: Instrument Bar

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 74 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.4. INSTRUMENT BAR Home Re-sets all parts to Home position – keeping all functions active Turning motors off Turns off all motors Calibrate probe height Calibrates Z maximum height Turning lights on Turns on/off internal lights...

  • Page 75: Settings

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 75 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.5. SETTINGS 4.5.1. HARDWARE CONFIGURATION The Xcalib requests a hardware configuration of the instrument which is going to be used to be input. All calibration procedures are disactived until the configuration is successfully loaded.

  • Page 76: Language

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 76 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.5.2. Language From “Settings” menu select “Language ”. Here choose which language is displayed: Italian English 4.5.3. Communication port From “Settings” menu select “Communication port”. The following display will appear: This display shows the possible active communication ports open by the PC from a pull down menu.

  • Page 77: Serial Number

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 77 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.5.4. Serial number From “Settings” menu select “Serial Number”. The following display will appear: Click on “Current value” to display actual number setting. To change serial number type in new number and click on “Store”...

  • Page 78: Work Area

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 78 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.6. WORK AREA This display shows the standard Work Area lay-out: 48 position sample rack Reagent and control rack Additional reagent rack Probe washing basin Strip holder incubation tray...

  • Page 79: Main Functions

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 79 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.7. MAIN FUNCTIONS 4.7.1. Tank level Sensors From “Main Functions” pull down menu select “Tank level sensors” to check that system is functioning correctly The following window is displayed: The condition of each tank will be automatically updated in some seconds and then displayed.

  • Page 80: Movement Test

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 80 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.7.4. Movement Test From “Main Functions” pull down menu select “Movement Test” to check that the different moving parts are functioning correctly This opens the following display: Click on “Start”...
  • Page 81 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 81 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 If one or more of the control results are negative an error message will be displayed specifying the problem. Here is an example of a negative test result where the camera has not been found – showing that communication with the camera is probably faulty: If “Dilutor Error: X-Axis movement failed!”...

  • Page 82: Print Preview

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 82 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.7.5. Print Preview From “Main Functions” pull down menu select “Print preview”. Print preview can only be selected to display the section of the Calibration function which is currently active. This function is only active when a calibration is taking place.

  • Page 83: Turn Light On

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 83 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.7.7. Turn Light On From “Main functions” pull down menu select “Turn light on” to check that internal lights are functioning correctly for reading phase If light does not come on check (and possibly substitute) the following - in order: connections are correct 2) Colight PCB.

  • Page 84: Dilutor

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 84 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8. DILUTOR 4.8.1. Calibrate probe positions From “Dilutor” menu select “Calibrate probe positions”. The following display will appear: Command Panel Work Area Any part of the Work Area can be selected with a mouse click on it. The probes will automatically move into the first position over the selected area.
  • Page 85 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 85 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Checking perpendicular positioning of probes 1 and 2 Turn off motors Position probe 1 over Work Area until it touches surface Check that probe 1 is perpendicular with tool PAM 26370-F as shown in following photo. When this procedure is completed return to Home position If it is not perpendicular then turn fixing screw on probe Z axis carriage until correctly perpendicular.
  • Page 86 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 86 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Calibration X-axis Calibration Centering the Dispensation Probe along X-axis over the Work Area is a mechanical calibration procedure where the OPTO PCB with the optic sensors is moved together with the APX-XGOLAV-X on the X carriage at the back of the device as shown in fig.
  • Page 87 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 87 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Y-Axis Calibration fig. 2 Y-axis calibration is a mechanical procedure which requires moving the encoder along the support ( Remove top cover Loosen screws at both ends of the encoder Move encoder manually until the probe is in the centre of the reference point and then tighten the screws Select “Go up”...
  • Page 88 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 88 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Aligning Shaking Plane Check Click on first strip holder incubation tray to select it (Tray 1) from Work Area display Select probe 2 from the command panel and then “Go down” command without changing the other two values (“Plate slope”...

  • Page 89: Calibrate Probe Height

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 89 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.2. Calibrate probe height From “Dilutor” pull down menu select “Calibrate probe height”. Height Calibration determines the safe distance between the probe tips and the bottom of the wells in each section of the Work Area–...
  • Page 90 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 90 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Incubation Tray Both Probe 1 and Probe 2 should be calibrated separately. Probe 1: Click on Work Area to select Strip Holder plate 1 and the first position is also automatically selected Type in “- 6”...

  • Page 91: Calibrate Lt

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 91 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.3. Calibrate LT From “Dilutor” pull down menu select “Calibrate LT” . The following display will appear: Check and Calibrate LT liquid sensor Ensure that sample rack heights have been correctly calibrated.
  • Page 92 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 92 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Setting LT values The report will display the following: sensitivity measurements “Probe 1 signal”: “Probe 1 height”: steps required to reach Z maximum height (to the liquid surface); Average values and CV% of measurements;...

  • Page 93: Calibrate Shaking Plane

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 93 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.4. Calibrate shaking plane From “Diluter” pull down menu select “Calibrate shaking plane” . The following display will appear: Control shaking plane movement Set up shaking range from pull down menu (default value is 6) and select “Start shaking”.
  • Page 94 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 94 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Check horizontal position: Click on “Move up” and then “Reset” to return shaking plane to the Home (flat) position • Use a spirit level to check that shaking plane is level horizontally along both X- and Y-Axis •...

  • Page 95: Calibrate Syringe

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 95 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.5. Calibrate syringe From “Dilutor” pull down menu select “Calibrate syringe”. The following display will appear: Calibration This procedure aims to regulate the distance between the syringe Tip and syringe mount is circa 1mm (fig. 1). syringe mount Syringe Tip Fig.
  • Page 96 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 96 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Set number of syringe movements in the “Tip Movement” box (between each movement there is a 3 second interval and the default movement setting is for 5 movements); Click on “Tip Position”;...
  • Page 97 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 97 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 To check aspiration and dispensation Visually check Waste tank is not full and that WASH basin is not empty. Select "Prime" to rinse/prime circuit. Set up Volume value for how much liquid should be aspired (default volume: 100µl).

  • Page 98: Calibrate Sul

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 98 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.6. Calibrate SUL From “Dilutor” pull down menu select “Calibrate SUL”. The following display will appear: SUL parameter settings regulate how long the dilutor unit valve remains open and consequently how much liquid volume (1 ml) is dispensed by the peristaltic pumps.

  • Page 99: Wash Basin Test

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 99 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.7. Wash basin test This test checks that the peristaltic aspiration/waste pumps and dilutor unit electro-valve are correctly functioning. From “Dilutor ” pull down menu select “Wash basin test” The following display will appear: Check peristaltic pump functioning Check that Waste basin is not full and that Wash basin is not empty.
  • Page 100 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 100 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Check correct functioning and resistance of electro-valve Check that probe valve is correctly functioning (open and close) through the two commands “Open probe valve” and “Close probe valve”...

  • Page 101: Prime

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 101 SECTION 5 Date: 02.04.2009 TROUBLE SHOOTING 4.8.8. Prime From “Dilutor” pull down menu select “Prime”. The following display will appear: Check that Waste basin is not full and that Wash and Wash Buffer 1 basins are not empty. Select type of prime: with WASH or with water (Wash Buffer1) If Buffer is selected then remove Wash Bottle cap If Wash is selected then remove top to Wash Buffer 1 bottle...

  • Page 102: Calibrate Camera

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 102 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.9. Calibrate camera From “Dilutor” pull down menu select “Calibrate camera” . The following display will appear: Fig. 1 Test correct gain calibration Check that internal light is on, instrument cover is closed and top cover is inserted.
  • Page 103 AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 103 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Gain Calibration Check that internal light is on and that instrument shield is closed and that upper cover is also inserted Select “Auto Gain ON”. The camera will then move to frame the Calibration Strip (as shown in Fig 1 ) and will automatically set a white balance.

  • Page 104: Calibrate Parameters

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 104 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 Save image The “Save image” command allows images displayed to be stored in a file with a *.bmp (bitmap) format. Camera settings The “Camera settings” command stores the camera own settings in a file with an *.ini format. Check PC performance The “Check PC performance”...

  • Page 105: Home

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 105 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.8.11. Home From “Dilutor” pull down menu select “Home” . Probes are returned to Home position over probe washing basin 4.8.12. Turning motors off From “Dilutor”...

  • Page 106: Instrument Information

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 106 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.9. ? 4.9.1. Instrument information From “? ” menu select “Instrument information” . The following display will appear: This display shows information about the firmware installed. If any function failure occurs then an Error message is displayed, e.g.: if communication with the instrument is not successful.

  • Page 107: Software Component Information

    AP Blot– SERVICE MANUAL Doc. N° MDS-AP-26-00-02 SECTION 4 Rev.01 Pag. 107 CALIBRATION PROCEDURES (XCALIB) Date: 02.04.2009 4.9.2. Software component information From “? ” menu select “Software component information”. The following display will appear: This display shows the different software versions in use. 4.9.3.

  • Page 109: Section 5 Trouble Shooting

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 109 SECTION 5 Date: 02.04.2009 TROUBLE SHOOTING SECTION 5 TROUBLE SHOOTING...

  • Page 111: Dilution / Dispensation

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 111 SECTION 6 Date: 02.04.2009 SCHEMATIC DIAGRAMS DILUTION / DISPENSATION PROBLEM PROBABLE CAUSE SOLUTION Liquid not dispensed Probe not in correct position Check motor, cable, and Dilutor card and replace parts as necessary. Probe goes into position but aspirates Check tubing, attachments and syringe air and then liquid cannot enter...

  • Page 112: Y - Z Movements

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 112 SECTION 5 Date: 02.04.2009 TROUBLE SHOOTING X - Y - Z MOVEMENTS ARM (X / Y AXES) PROBLEM PROBABLE CAUSE SOLUTION X-Y arm does not stop moving Defective optic sensor Replace XGOLAV X or XGOLAV Y opto card as appropriate.

  • Page 113: Section 6 Schematic Diagrams

    AP Blot – SERVICE MANUAL Doc. N° MDS-AP-26-00-02 Rev.01 Pag. 113 SECTION 6 Date: 02.04.2009 SCHEMATIC DIAGRAMS SECTION 6 SCHEMATIC DIAGRAMS...

Table of Contents