Page 1 H ITACH I U LTRAC EN TR IFUG E CP100NX／90NX／80NX INSTRUCTION MANUAL －Important－ Before using this CENTRIFUGE carefully read through this INSTRUCTION MANUAL to ensure efficient, safe operation. Keep this INSTRUCTION MANUAL available as an important reference when using the CENTRIFUGE.
Page 3 Copyright © 2015 Hitachi Koki Co., Ltd. All rights reserved. No part of this document may be reproduced or transmitted in any form or any means without permission from Hitachi Koki Co., Ltd. The names of actual companies and products mentioned herein may be the trademarks of their respective owners.
Page 5 This series offers many new features that we are confident of satisfying your requirements. These features include the following. 1. Maximum speed is 100,000 rpm(803,000 x g).(CP100NX) 2. When using RLM rotors, the rotor life time is automatically managed by the rotor life management (RLM) feature of the ultracentrifuge, so keeping a rotor log book is not necessary.
Page 6 "NOTE" indicates a note which has no direct bearing on personal safety. Do not perform any operation not specified in the instruction manual. If any problem is found on your instrument, contact a Hitachi Koki authorized sales/service representative. Although the safety precautions in the instruction manual and safety instructions on your instrument have been fully considered, an unexpected situation may arise.
Page 7 Use a RLM rotor manufactured by Hitachi Koki with only Hitachi ultracentrifuges. ● Failure to do so can cause the centrifuge to become damaged.
Page 8 After installation and before any test-run, this ultracentrifuge always needs ● the internal check by a Hitachi Koki authorized sales/service representative. Repairs, disassembly, and other modifications to the centrifuge are strictly ●...
Page 9 Hitachi Koki authorized sales/service representative. Notice for an Earthquake Depending on the magnitude, an earthquake might damage centrifuge. If you observe some abnormality, stop using the centrifuge immediately and ask for inspection by the Hitachi Koki service representative.
Page 10 2.Indication of WARNING Use a RLM rotor manufactured by Hitachi Koki with only Hitachi ultracentrifuges. Failure to do so can cause the centrifuge to become damaged.(Section2-2-4) 1. Do not remove the RLM adapter or optical adapter from the rotor, or replace it with the adapter for another rotor.
Page 11 (Chapter4 and Chapter5) Unspecified repairs, remodeling or disassembly of the centrifuge that is not listed in Table 5-1 is strictly prohibited by any person other than a Hitachi Koki authorized sales/service representative. (Section5-1) Before changing the power voltage by manually selecting desired tapping on the internal transformer, turn off power to the ultracentrifuge, then unplug the power cord from the wall outlet.
Page 12 (Section3-1) 1. Do not press the touchscreen with a sharp-pointed object such as a ballpoint pen. 2. If abnormal sound or vibration occurs, stop the operation immediately and contact a Hitachi Koki authorized sales/service representative. (Section3-2) If you have selected a rotor with an optical disk in the Rotor Management screen, be sure to check that the selected rotor (in the Rotor Management screen) and the rotor that is installed in the centrifuge have an identical serial number.
Page 13 Using cleaning or sterilization method other than recommended in this instruction manual may cause corrosion or deterioration of this centrifuge. Refer to chemical resistance chart attached to the rotor or contact Hitachi Koki. (Chapter4) For sterilization of the surface of the centrifuge and the rotor chamber, wipe them with a cloth dampened with 70% ethanol.
Page 14 SAFETY NOTICES WARNING Never use any material capable of producing flammable or explosive vapors. Be sure to take the necessary precautions before separating radioactive, toxic or pathogenic materials. AVERTISSEMENT Ne jamais utiliser de matériau capable de produire des vapeurs inflammables ou explosives. Assurez-vous de prendre les précautions nécessaires avant de trier des matières radioactives, toxiques ou pathogènes.
Page 15: Table Of Contents
Table of Contents 1. Specifications ······························································································ 1-1 2. Description ·································································································· 2-1 2-1 External View of Ultracentrifuge ································································· 2-1 2-2 Structure ······························································································· 2-2 ··················································· 2-2 2-2-1 Touchscreen and External Connection ················································································ 2-6 2-2-2 Rotor Chamber ················································································ 2-7 2-2-3 Safety Devices 2-2-4 Rotor Adapter/Disk ···········································································...
Page 16 Table of Contents ··············································································· 3-84 3-4-6 Admin Function ········································································ 3-85 (1) User management ··············································································· 3-90 (2) User lockout (3) Rotor management ······································································· 3-92 ······································································· 3-98 (4) Run time indication ··········································································· 3-98 (5) Actual run timer ·············································································· 3-99 (6) Vacuum level ···············································································...
Page 17: Specifications
1. Specifications 1. Specifications Model CP100NX CP90NX CP80NX Maximum speed 100,000 rpm 90,000 rpm 80,000 rpm 803,000 xg 700,000 xg 615,000 xg Maximum RCF* (P100AT2) (P90AT) (P80AT) ±2rpm（1,000rpm to maximum speed） Speed control accuracy 10-stage variable acceleration control, 10-stage braked deceleration...
Page 18 • ** The voltage to be used is the one that you specified when purchasing the centrifuge. The CP100NX/90NX/80NX ultracentrifuges satisfy CE marking requirements. The CE marking is an international symbol which shows that the product conforms to EC directives.
Page 19: Description
The CP-NX Series Ultracentrifuges are floor-standing types. These three types of ultracentrifuges have the same external view, except for the model name printed on front cover and the same dimensions. The following is the external view of the CP100NX Ultracentrifuge. Unit: (mm)
Page 20: Structure
2-2 Structure 2-2-1 Touchscreen and External Connection 2-2 Structure 2-2-1 Touchscreen and External Connection The touchscreen with color liquid crystal screen is incorporated in the CP-NX Series. You can set the run parameters, perform the operation, and display a run history, programmed operation, and user customizations screens by pressing the screen.
Page 21 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Name and symbol Functions and actions These areas display various types of information. Display areas The SPEED (RCF), TIME, and TEMP areas display the current status in the upper part and the specified setting in the lower part.
Page 22 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Functions and actions Name and symbol ● The following four stages are displayed, depending on the vacuum of the rotor chamber. Atmospheric state. （1） The vacuum pump is not active. Low vacuum.
Page 23 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Functions and actions Name and symbol On-screen keypad Use the on-screen keypad to enter numeric values for run ● parameters. [ : / . ] When entering a time: Switches from hours to minutes [Hold] When entering the operation time: Sets continuous running.
Page 24: Rotor Chamber
2-2-2 Rotor Chamber 2-2-2 Rotor Chamber The structure of the rotor chamber (vacuum chamber) is shown in Fig. 2-2-2. Bowl (Rotor Chamber) Temperature sensor (measuring Drive shaft (crown) the temperature of the rotor) Handle Chamber door seal Chamber door Speed sensor Overspeed detector Door lock system (detecting any instance...
Page 25: Safety Devices
2-2-3 Safety Devices 2-2-3 Safety Devices (1) Protection of rotor chamber Should the rotor spinning at high speed fails (or comes off the drive shaft), the safety of the operator is ensured by the thick protective steel ring enclosing the bowl (Fig. 2-2-2). (2) Imbalance detector If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper bucket setting, the imbalance detector detects the situation and decelerates the rotor immediately.
Page 26: Rotor Adapter/Disk
RLM adapters are termed RLM rotors (automatically managed rotors.) (See Fig. 2-2-3). WARNING: Use a RLM rotor manufactured by Hitachi Koki with only Hitachi ultracentrifuges. Failure to do so can cause the centrifuge to become damaged.
Page 27 2-2-4 Rotor Adapter/Disk You should note that there are two versions of the CP-NX Series ultracentrifuges: One is designed for rotors having optical adapters and the other, for rotors having optical disks. The RLM rotors can be used on both types, but rotors having optical adapters cannot be used on the ultracentrifuge version designed for rotors having optical disks and vice versa.
Page 28: Operation
3. Operation 3. Operation The centrifuge comes with a wide range of functions to support a broad range of applications. A brief description of each mode of operation is given below. Brief description Reference Section 3-2 "Basic Operation" Normal operation Time You can save set run parameters in memory for later use in repeated operation.
Page 29 3. Operation Brief description Reference This function is used to perform a run between a set start time and end time. Section 3-3-4 "RTC End of set Operation" Press the run time RTC operation START （Real Time Control） button Time [Special operation] Brief description Reference...
Page 30: Run Preparation
3-1 Run Preparation 3-1-1 Starting up the centrifuge 3-1 Run Preparation WARNING: (1) This centrifuge is not explosion proof centrifuge. Never use explosive or flammable samples or materials that chemically react vigorously. Do not centrifuge such materials in this instrument nor handle or store them near the instrument.
Page 31: Rotor And Tubes Preparations And Precautions
3-1-2 Rotor and Tube Preparations and Precautions 3-1-2 Rotor and Tube Preparations and Precautions The CP-NX series centrifuges allow you to balance samples, by eye. The difference between meniscus levels of opposing samples must be less than 5 mm (Fig. 3-1-2), but some rotors are exceptions.
Page 32: Basic Operation
CAUTION: (1) Do not press the touchscreen with a sharp-pointed object such as a ballpoint pen. (2) If abnormal sound or vibration occurs, stop the operation immediately and contact a Hitachi Koki authorized sales/service representative. 3-2-1 Setting Run Parameters This section will describe the Run screen, the starting point of basic operations. For information on screens displayed during normal operation and entry of run parameters, refer to Section 2-2-1 "Touchscreen and External Connections."...
Page 33 3-2-1 Setting Run Parameters [Display and operations when entering run parameters] Press the SPEED, TIME, TEMP, ACCEL or DECEL field on the touchscreen to display the on-screen keypad. (1) Press the field of the item you want to set. The initial digits are displayed in blue.
Page 34 3-2-1 Setting Run Parameters Methods for setting run parameters are described on the next page. Note 1) If you enter the wrong value, press the [CE] button and enter the correct value. If you have already pressed the [Enter] button, repeat the process and enter the correct value.
Page 35 3-2-1 Setting Run Parameters  Setting speed, run time and temperature Operation procedures will be described using examples. Speed run time Temperature Setting Item （SPEED）（TIME）（TEMP） °C Setting example 100,000 rpm 2 hours 30 minutes Press the SPEED, TIME or TEMP field to display the on-screen keypad.
Page 36 3-2-1 Setting Run Parameters  Setting acceleration and deceleration mode Acceleration Deceleration Setting Item （ACCEL）（DECEL） Setting example Press a field to display the on-screen keypad. Press the field whose value you want to set. The numeric values are displayed in blue. Color: Blue Color: Blue Press the on-screen...
Page 37: Acceleration And Deceleration Modes
3-2-2 Acceleration and Deceleration Modes 3-2-2 Acceleration and Deceleration Modes In order to meet a wide variety of applications, the acceleration and deceleration rates can be adjusted between 0 to 500 rpm. By setting an ideal acceleration and deceleration time samples will not be disturbed, which improves efficiency and reduces centrifugation time.
Page 38: Rotor Selection
3-2-3 Rotor Selection 3-2-3 Rotor Selection This centrifuge stores the maximum and average radius of each rotor in internal memory. It provides a function that will automatically calculate and display the relative centrifugal force (RCF) from a set speed and calculate and display the speed from an RCF setting (for information, refer to section 3-3-2 "RCF display and setting function").
Page 39 3-2-3 Rotor Selection Step Touchscreen operation Screen displays and notes When the Rotor management ● The ROTOR screen does not display the CATALOG screen desired rotor, press the [Rotor Catalog] button. In the ROTOR CATALOG screen, select the desired rotor type. In the screen displaying rotor ●...
Page 40 3-2-3 Rotor Selection Step Touchscreen operation Screen displays and notes The Run screen appears and The Run screen ● the name of the selected appears. rotor type is displayed in the Rotor indicator field. Rotor indicator field NOTE (1) You cannot perform life management of a rotor equipped with an optical disk just by selecting a rotor in the ROTOR CATALOG screen.
Page 41: Logging In As A User
3-2-4 Logging in as a User 3-2-4 Logging in as a User By registering users in the instrument, it will be possible to manage the operation history of users that log in. For information on how to register a user, refer to Section 3-4-6 (1) "User Management." User Login Procedures Step Touchscreen operation...
Page 42 3-2-4 Logging in as a User Step Touchscreen operation Screen displays and notes The Run screen ● The user name appears in the appears. USER area in the Run screen. USER area NOTE 1) When the user lockout function described in Section 3-4-4 (2) "User Lockout" is enabled, you must login as a user to operate the centrifuge.
Page 43: Operation Procedures
3-2-5 Operation Procedures 3-2-5 Operation Procedures Below is a description of normal operating procedures. CAUTION: Take care not to get your hands or fingers caught in the door of the centrifuge. Before you start using your centrifuge, carefully read through the instruction manual for your NOTE rotor and make sure that the correct tube types and number of samples are supplied.
Page 44 3-2-5 Operation Procedures Step Touchscreen operation Centrifuge operations and notes The [START] button lamp blinks and the rotor starts turning.  Press the [START] button. The timer begins operating. (If the actual run timer is set, the  timer will start operating when set speed is reached.) When the set speed is reached, the [START] button lamp ...
Page 45 3-2-5 Operation Procedures Fig. 3-2-3 is an overview of the procedures described above. See Section 3-4-3 "Zonal Operation Procedures" for information on using a zonal rotor. Start of operation Turn on the : Indicates switch or button. centrifuge POWER Open the door and install the rotor.
Page 46: How To Use The Function Selection Area
3-3 How to Use the Function Selection Area 3-3 How to Use the Function Selection Area This centrifuge provides programmed operation including step-mode operation, RCF display and setting function, ω T display and setting function and Real Time Control (RTC) that can run the centrifuge at a preset date and time and a host of other functions.
Page 47: Programmed Operation
3-3-1 Programmed Operation 3-3-1 Programmed Operation When a centrifugal condition will be used frequently, entering the same condition every time you want to perform centrifugation is inconvenient. This centrifuge has a programmed operation feature that stores run parameters. Storing frequently used run parameters allows you to call those parameters when you need them, which saves setup time.
Page 48 3-3-1 Programmed Operation (Basic operation of the programmed operation feature) Press the [PROGRAM] button. Program Step operation display display screen The Function Selection Area changes to the Program display screen. [PROGRAM No.] button [Folder] button If the registered program If the registered program No.
Page 49 3-3-1 Programmed Operation As the Run screen appears, ● the registered run parameters are set. program No. is displayed ● in the [Folder] button in the Program display screen. ● When multiple steps are registered, the step operation display indicates the total number of steps and the current step.
Page 50: Program Registration Procedures
3-3-1-1 Program Registration Procedures 3-3-1-1 Program Registration Procedures (1) Procedures for registration of run parameters Procedures for registering run parameters are described below. Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen ● appears. POWER switch. [PROGRAM] button Press the [Program] button ●...
Page 51 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the [Folder] button in The Run screen ● replaces the the program display screen. Program list screen. Program folders ● come in four colors. The program list can ●...
Page 52 3-3-1-1Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the [New Folder] The Program No. ● entry screen appears. button. Program No. entry field The unregistered To register the folder, press program with the the [Enter] button. To select lowest number is a different number, use the displayed when the...
Page 53 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the white area in the ● The User Management Registered user screen appears. display/registration field ((2)) to select a user. (You can omit this operation.) Select the user name you want to use and press the [Select] ...
Page 54 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the field in Step 1, check that the field turns blue and press the [Edit] button. [Edit] button run parameter ● Press the white area in the entry/display screen SPEED setting value display appears.
Page 55 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The run parameter ● Check the entry and press entry/display screen the [OK] button. reappears. The SPEED, TIME, ● TEMP display fields and the on-screen keypad appear. Selecting a rotor in step ●...
Page 56 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The RCF setting screen ● Press the display area of the appears. item you want to set and Set rotor model enter the desired value. Example) SPEED display RCFmax：513,700×g RCFavg setting value display field RCFmax setting value...
Page 57 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes ω Press the ω setting ● T display area screen appears. and enter the desired value. Example) ω T：1.98×10 ω display (Left area) Mantissa (Right area) Exponent Enter the desired value and press the [Enter] button. ...
Page 58 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Check the setting and press When a normal operation is registered the [Register] button. The Program list ● screen appears. Program registered in this example When a step-mode operation is registered The Check ●...
Page 59 3-3-1-1 Program Registration Procedures (2) Procedures for Changing Program run parameters The following describes how to change run parameters for registered programs. Step Touchscreen operation Screen displays and notes Press the [PROGRAM] button in the Run screen (in the Function Selection Area). [Folder] button [PROGRAM] button Program display screen...
Page 60 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes ● The run parameter To change run parameters, setting/display screen press the [Next] button. appears. [Edit] button Press a field in Step 1, check The run parameter ● entry/display screen that the field turns blue and appears.
Page 61 3-3-1-1 Program Registration Procedures (3) Procedure for deleting programs The following describes how to delete registered programs. Step Touchscreen operation Screen displays and notes Open the Program list The program list ● appears and the screen and press the frame of the selected program folder you want to folder turns blue.
Page 62 3-3-1-1 Program Registration Procedures (4) Operation procedures for copying programs The following describes how to copy a registered program and register the result as a new program. This is a convenient method when you want to register a new program by changing part of a registered program.
Page 63 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes ● The Program list Press the [Enter] button. screen appears. (The [Enter] button will not accept the entry of an already registered program No.) The copied and newly registered program folder The copied program is registered under a new number.
Page 64: Programmed Operation Procedures
3-3-1-2 Programmed Operation Procedures 3-3-1-2 Programmed Operation Procedures The following describes procedures for "programmed operations" by calling registered run parameters. This is convenient for using the same run parameters repeatedly. (1) If the registered program no. is known Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen appears.
Page 65: Step-Mode Operation Procedures
3-3-1-2 Programmed Operation Procedures Step Touchscreen operation Screen displays and notes The number of the called program is displayed in the  Program display screen. It allows you to set and display run parameters (SPEED,  TIME, TEMP, ACEL/DECEL, ROTOR, USER). ...
Page 66 3-3-1-2 Programmed Operation Procedures (2) If the registered program no. is not known Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen appears. POWER switch. The Function ● Press the [PROGRAM] Selection Area button in the Function changes to the Selection Area.
Page 67 3-3-1-2 Programmed Operation Procedures Step Touchscreen operation Screen displays and notes b-1) To confirm registered ● The MENU box appears in the Program list data, press the [Folder] screen. button you want to verify, and press the [MENU] button after the folder Reference button turns blue.
Page 68 3-3-1-3 Step-Mode Operation Procedures 3-3-1-3 Step-Mode Operation Procedures This centrifuge is provided with a "step-mode operation" function that can store two or more run parameters in one program memory area and a total of 30 steps. This allows it to change speed, run time, temperature and other conditions during operation.
Page 69 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes  Program registration screen  Run parameter setting/display As described in steps 1 to 8 in "(1) Procedures for registration of run parameters" in Section 3-3-1-1 "Program registration procedures," enter the Program No., registered rotor, user and remarks, select a folder color...
Page 70 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes The run parameter ● Press the [Next Step] button entry/display screen and enter the run in STEP 3 appears. parameters for step 3 from the run parameter entry/display screen for step STEP 3 [Register] button SPEED: 60,000 rpm...
Page 71 3-3-1-3 Step-Mode Operation Procedures (2) Step-Mode Operation Procedures Call the number of the program where the step-mode operation is registered and run it according to the instructions in Section 3-2-5 "Operation Procedures." Start operation without changing the run parameters after calling the program. (Changing the run parameters will cancel the called program.) NOTE 1) The Program display screen in the Run screen displays the following data.
Page 72 3-3-1-3 Step-Mode Operation Procedures (3) Procedures for Registering Step-Mode Operation that Includes Stops Follow the instructions in "(1) Registration Procedures for Step-Mode Operation." Perform the registration procedures described below. [Setting example] The following describes the setup procedures for continuous operation under the five run parameters including a 0 rpm settings shown in the figure below.
Page 73 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes Enter the run parameters for The run parameter ● step 1 as described in "(1) entry/display screen Registration Procedures for appears. Step-Mode Runs." [Next Step] button STEP 1 SPEED: 50,000 rpm TIME ：1 hour TEMP ：15.0 °C ACCEL: 9...
Page 74 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes ● The Program list Check the entries displayed screen reappears. for each step in the run parameter setting/display [X] button screen. If they are correct, press the [X] button and press the [X] button in the The program Program registration screen.
Page 75 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes The Program display ● When run time for step 1 has screen in the Run elapsed, processing proceeds screen proceeds to to step 2 and the rotor step 2. decelerates and stops.
Page 76: Rcf (Relative Centrifugal Force) Display And Setting Function
3-3-2 RCF Display and Setting Function 3-3-2 RCF (Relative Centrifugal Force) Display and Setting Function This centrifuge stores the maximum and average radii of each rotor in internal memory. Setting a speed causes the centrifuge to automatically calculate and display the RCFmax value (the maximum centrifugal force of each rotor) and the RCFavg value (the average centrifugal force of each rotor).
Page 77 3-3-2 RCF Display and Setting Function Step Touchscreen operation Screen displays and notes Set rotor model Press the RCF display screen The RCF setting ● field. screen appears. SPEED display Calculated RCFavg value Calculated RCFmax value  The RCF setting screen displays the RCFmax and RCFavg values computed from the currently displayed SPEED value and the rotor set in step 1.
Page 78 3-3-2 RCF Display and Setting Function Step Touchscreen operation Screen displays and notes Check the entered value and ● The entered value is confirmed. press the [Enter] button. SPEED display Calculates and displays the RCFavg value from the  entered RCFmax and the rotor set in step 1 (the value here is 355,000 x g).
Page 79: Ω 2 T Operation
3-3-3 ω T Operation 3-3-3 ω T Operation This function is used to indicate ω T, (a value obtained by adding run time to the angular velocity squared) the centrifugal effect. When the same rotor is used, setting the ω T makes it possible to produce centrifugation that is easily reproducible.
Page 80 3-3-3 ω T Operation Touchscreen operation Screen displays and notes Step Press the on-screen keypad to enter a setting value. When the mantissa has been entered, press the exponent entry field, enter the Exponent exponent and press the entry field [Enter] button.
Page 81: Rtc (Real-Time Control) Operation
3-3-4 RTC (Real Time Control) Operation 3-3-4 RTC (Real Time Control) Operation This centrifuge contains an internal clock allowing you to operate the centrifuge between a set start time and end time. This function for operating the centrifuge between set time intervals is called Real Time Control (RTC).
Page 82 3-3-4 RTC (Real Time Control) Operation Step Touchscreen operation Screen displays and notes The setting value is Set run parameters ● displayed in the according to the instructions setting value display in Section 3-2-1 Setting run field. parameters. Setting value display field [RTC] button ...
Page 83 3-3-4 RTC (Real Time Control) Operation Step Touchscreen operation Screen displays and notes To set the start time, press the [Start Time] button and to set the stop time, press the [Stop Time] button. Enter the Set state display start time and stop time from the on-screen keypad.
Page 84 3-3-4 RTC (Real Time Control) Operation NOTE 1) In the following situations RTC cannot be set. (1) When TIME (run time) is set to HOLD (continuous operation) in the Run screen Change the run time (centrifugation time) to a numeric value instead of HOLD.
Page 85: Features Of The Menu Screen
3-4 Features of the MENU Screen 3-4 Features of the MENU Screen These features have been designed to make your CP-NX Series ultracentrifuge easier to use by including, for example, the Run History function shown in Fig. 3-4. The MENU screen can be displayed by pressing the MENU tab on the touchscreen. MENU tab Total number of runs for this...
Page 86 3-4 Features of the MENU Screen Name Icon Function Starts the Defrost function that heats the rotor chamber to quickly Defrost remove frost and moisture. (For details, refer to Section 3-4-4 function "Defrost ") Allows you to adjust screen displays, the stop signal and other Customize settings in a way that works best for you.
Page 87 3-4-1 Outputting and Reusing Run History Display and Run Parameters 3-4-1 Outputting and Reusing Run History Display and Run Parameters The centrifuge can automatically store a run history containing up to 5,120 run parameters. The run history can be used for checking operation and for reuse, verify user and rotor performance and output data in the CSV format.
Page 88 3-4-1 Outputting and Reusing Run History Display and Run Parameters Step Touchscreen operation Screen displays and notes The touchscreen displays the Run screen again and the  Press the [Load for a new run parameters in the history are set. run] to use the run parameters in the Run history details screen.
Page 89 3-4-1 Outputting and Reusing Run History Display and Run Parameters (2)-2 Checking and Using User Run History Step Touchscreen operation Screen displays and notes Press the [Menu] button at the ● The Menu box in Run History appears bottom left in the Run History screen.
Page 90 3-4-1 Outputting and Reusing Run History Display and Run Parameters (2)-3 Checking and Using Rotor Run History Step Touchscreen operation Screen displays and notes The Menu box in Run ● Press the [Menu] button at the History appears bottom left in the Run History screen.
Page 91: Rotor Catalog
3-4-2 Rotor Catalog 3-4-2 Rotor Catalog This catalog provides information on rotor models, rotor specifications and rotor accessories like tubes, caps and adapters. (1) Example showing how to referencing the catalog Step Touchscreen operation Screen displays and notes Press the [Rotor Catalog] The ROTOR ●...
Page 92 3-4-2 Rotor Catalog Step Touchscreen operation Screen displays and notes The Tube list Pressing the [TUBE LIST] ● screen appears. button opens the Tube list screen, which shows tubes available for the selected rotor. Press the [<] and [>] buttons to turn pages.
Page 93: Zonal Operation Procedures
Use of rotors other than zonal rotors will lead to small deviations in temperature indication. If you intend to use the P32CT continuous flow rotor (a black-painted rotor) for zonal operation, contact a Hitachi Koki sales/service representative.
Page 94 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes Press the [ZONAL] button in The Set Validation ● the ZONAL setting screen. No. screen appears. Enter “30” for the PIN on the ZONAL operation is ● on-screen keypad and press enabled when the [ZONAL] button is the [Enter] button.
Page 95 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes The selected rotor Press the Rotor indicator field ● type appears in the to select a zonal rotor as Rotor indicator field. described in Section 3-2-3 "Rotor Selection." Rotor indicator field The rotor accelerates to zonal speed (normally 3,000 rpm) ...
Page 96 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes The set time elapsed. (Time When the set time elapses, the rotor decelerates and  out) stabilizes at the zonal speed. The zonal operation chart uses a steady or blinking red light ...
Page 97: Defrost
3-4-4 Defrost 3-4-4 Defrost The presence of frost or moisture in the rotor chamber (especially in the bowl) prior to a run will considerably lengthen the time it will take to reach a high vacuum level. This centrifuge comes with a defrost function that heats and evacuates the bowl to quickly remove frost and moisture.
Page 98 3-4-4 Defrost Reference As described below, the defrost function operates differently depending on centrifuge status when defrosting is started. Evacuation is performed simultaneously with defrosting. Enabling defrosting when the rotor is Enabling defrosting when the rotor is standing still rotating Defrosting starts when selected and Defrosting starts when rotor speed starts continues for 10 minutes.
Page 99: Customizing
3-4-5 Customizing 3-4-5 Customizing This function allows you to adjust Run screen displays, the stop signal, sound volume, screen backlight brightness and other functions to your preferences. Press the [Customize] button in the MENU screen to open the CUSTOM screen with the icons for setting and changing zoom display, the stop signal and other functions shown in Fig.
Page 100: Zoom Display Setting
3-4-5 Customizing Name Icon Function Use for making date and time settings. Date/Time (For details, refer to Section 3-4-5 (7)) Status display Use to set status displays (LED indicator). lamp (For details, refer to Section 3-4-5 (8)) Sets economy mode (auto standby mode). (For details, refer Economy mode to Section 3-4-5 (9)) Press the icon for the function you want to use and select from the options that appear.
Page 101: Sound Volume Adjustment
3-4-5 Customizing (3) Sound volume adjustment Adjusts the sound volume of the stop signal. More green segments in the indicator bar means a larger sound volume. The stop signal does not sound when all segments in the indicator bar are black. Use the sound volume adjustment buttons as described below.
Page 102: Schedule
3-4-5 Customizing  Pressing when the dimming backlight mode is enabled opens the screen shown in Fig. 3-4-5 (4c) that allows you to set the time when the dimming backlight mode will be engaged. You can set a time between 1 and 180 minutes in one minute increments.
Page 103 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the [Add] button. The User list ● screen appears. User cell  Use the [<] and [>] buttons to turn pages in the user list Press the cell of the user The User PIN field ●...
Page 104 3-4-5 Customizing 2) Method for canceling centrifuge schedules Step Touchscreen operation Instrument operations and notes Press the [Schedule] button. ● The CUSTOM screen is replaced by the Schedule screen. ● The selected cell Press the schedule cell you turns blue. want to delete.
Page 105: Date/Time Setting
3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Enter the PIN of the user who ● The Schedule list screen appears. registered the schedule and press the [Enter] button.  The selected schedule is deleted from the schedule list. Press the CUSTOM tab, the [X] button, the Run screen tab ...
Page 106: Status Display (Led Indicator) Lamp Setting
3-4-5 Customizing (8) Status display (LED indicator) lamp setting Sets the display color, brightness and lighting pattern of the status display lamp (LED indicator) beside the rotor chamber door to indicate instrument operating conditions. The display color, brightness and display pattern of the status display lamp can be set for the operating conditions described below.
Page 107 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the [Set display The Color palette ● screen appears. color] button for the operating condition you want [X] button to change. [Select display color] button Example: To change the display color for [When stopped] from blue to [SET] button white...
Page 108 3-4-5 Customizing 3) 2 Method for setting display patterns for the status display lamp Step Touchscreen operation Instrument operations and notes Press the [LED] button. ● The LED indicator setting screen appears. [Set pattern] button Press the [Set pattern] ● The Pattern palette screen appears.
Page 109: Economy Mode Setting
3-4-5 Customizing (9) Economy mode setting When economy mode is enabled, the touchscreen backlight will automatically dim and the cooling fan will stop turning to reduce power consumption when the centrifuge has been left idle for a preset period of time. 1) Method for engaging economy mode Step Touchscreen operation...
Page 110 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the CUSTOM tab or the [X] button to return to the  Press the "CUSTOM" tab, the CUSTOM screen. [X] button, the Run screen Press the Run screen tab or MENU tab to return to the ...
Page 111: Admin Function
3-4-6 Admin Function 3-4-6 Admin Function This function allows you to access administrator functions. Press the [Admin] button in the MENU screen to open the ADMIN screen shown in Fig. 3-4-6. This screen provides icons for controlling functions like user management and user lockout. Screen tabs Fig.
Page 112: User Management
3-4-6 Admin Function Name Icon Function Allows you to set the vacuum conditions when the centrifuge starts accelerating from vacuum standby Vacuum level state to the set speed. (For details, refer to Section 3-4-6 (6)) Zonal speed can be changed in increments of 100 rpm Zonal speed between 2,000 and 3,000 rpm.
Page 113 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The buttons in the Register user name screen have the  following functions. [Shift] button: Press to switch from lower case to upper case letters. [Clear] button: Deletes all characters in the User name field.
Page 114 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes Again enter the PIN entered ● An Access level dialog box appears. in step 5 and press the [Enter] button. Access level dialog box An access level setting makes it possible to control access at ...
Page 115 3-4-6 Admin Function 2) Method for deleting users and changing registered information Step Touchscreen operation Screen displays and notes Press the [User The Admin screen ● replaces the user Management] button. management screen. Press the User field of the ● The selected user field changes from user you want to delete or...
Page 116 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes 4 -2 Press the [Change the The Select access ● level field appears. Access level] button to change the access level. Access level dialog Enter the user PIN you want to change and press the [Enter] ...
Page 117: User Lockout
3-4-6 Admin Function (2) User lockout User lockout when enabled, makes it possible to control user access at three different levels. 1) Method for setting user lockout Step Touchscreen operation Screen displays and notes  The user that logs in must have [Administrator] privileges. Log in as a user as described in Section 3-2-4 "Logging in as a User."...
Page 118 3-4-6 Admin Function NOTE 1) Only a user logged in with [Administrator] privileges can enable the user lockout function. 2) Use of the user lockout function requires that users are registered. Register users as described in (1) "User Management." 3) When user lockout is enabled, a user must be logged in to use the touchscreen. 4) Once user lockout is enabled, only a user with an Administrator access level can call the Admin screen.
Page 119: Rotor Management
3-4-6 Admin Function (3) Rotor management If you have a rotor with an optical disk (refer to Section 2-2-4 "Rotor Adapter/Disk"), you can register the rotor and its serial number in the instrument to use it in setting operations and control total run time and number of runs. Rotor life management is essential in operating an ultracentrifuge.
Page 120 3-4-6 Admin Function 3) Method for managing rotor life data (1) For rotors with an RLM adapter used in multiple CP-NX centrifuges (automatic life management) Case example Data stored in RLM adapter memory CP-NX No. 1 Total data for use in No. 1: A Data A CP-NX No.
Page 121 3-4-6 AdminFunction 4) Procedures for registering a rotor with an optical disk (1) Procedure for registering a new rotor with an optical disk (This procedure is not required with rotors with an RLM adapter.) Step Touchscreen operation Screen displays and notes The Rotor ●...
Page 122 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The Rotor ● Enter the serial number (S/N) Management screen and press the [Enter] button. appears. Enter a four digit serial number, if the serial number is "43," prefix it with 0 and enter "0043."...
Page 123 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The Number of runs ● Press the [Edit Runs] button field appears. in the menu box. From the "Rotor log" The Rotor ● check the total number of management screen runs the rotor was used in the reappears and the other centrifuge, enter it and...
Page 124 3-4-6 Admin Function (3) Procedure for deleting a registered rotor Step Touchscreen operation Screen displays and notes Press the Rotor management ● The MENU box appears in the Rotor button, select the rotor to management screen. delete and press the [MENU] button.
Page 125: Run Time Indication
3-4-6 Admin Function (4) Run time indication Select either the elapsed time or remaining time as run time. Note that when the run time setting is set to [HOLD], elapsed time is automatically selected. 1) [Elapsed] button: Elapsed time is displayed in the centrifuge run time indication field in the Run screen and "Lapse"...
Page 126: Vacuum Level
3-4-6 Admin Function (6) Vacuum level You can select a vacuum level when acceleration starts after exceeding the vacuum waiting time at 4,000 rpm. Select Start high vacuum to prevent a temperature rise caused by wind. 1) [Medium] button: When the vacuum level in the rotor chamber reaches about 133 Pa, the vacuum waiting time ends and the rotor starts to accelerate to reach the set speed.
Page 127: Lan Communications
3-4-6 Admin Function (8) LAN communications Connects and disconnects LAN communications. When the centrifuge is connected to a LAN, the "himac LogManager" software can be used for communications and storing operation results. To connect to a LAN, insert a LAN cable into the LAN port in the external connections on the right side of the instrument.
Page 128: Instrument Id, Service Contact Information
3-4-7 Instrument ID, Service Contact Information 3-4-7 Instrument ID, Service Contact Information When multiple instruments are used, set separate IDs in each. Service contact information and other data can be stored as memos. Step Touchscreen operation Screen displays and notes Press the [ID/Contact] ●...
Page 129 3-4-7 Instrument ID, Service Contact Information Step Touchscreen operation Screen displays and notes Press the MENU tab or the [X] button to return to the MENU  Press the MENU tab, [X] screen. button or the Run screen tab. Press the Run screen tab to display the Run screen. ...
Page 130: Happenings When Power Failure Occurs
(2) Never touch the rotor during rotation. CAUTION: Do not perform any operation not specified in this manual. If any problem is found on your centrifuge, contact a Hitachi Koki authorized sales/service representative. 1. Rotation of the rotor The rotor coasts to a stop. If the power is restored when the current speed is more than 500 rpm, the rotor will be automatically accelerated to the set speed.
Page 131 3-5 Happenings when Power Failure Occurs (2) Turn off the POWER switch and power circuit breaker for the ultracentrifuge. (3) Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the front cover by pulling its lower side forward. Then, remove the front cover. The upper side of the front cover is an insertion type.
Page 132 3-5 Happenings when Power Failure Occurs Handle Vacuum release screw Vacuum chamber Power breaker Door unlock wire Fig. 3-5-2. Ultracentrifuge with the front cover removed M5 hexagon head bolt for fixing the front cover (4 pcs.) Fig. 3-5-3. Installing the front cover (7) Turn on the power breaker.
Page 133: Maintenance
Hitachi Koki may ask you about the treatment for the centrifuge, rotor or the part if the decontamination is checked and judged as insufficient by Hitachi Koki. It is your responsibility to bear the cost of sterilization or decontamination.
Page 134: Rotor Chamber
70% ethanol. Using the method other than the above method might cause corrosion or deterioration of the centrifuge. Refer to the chemical resistance chart provided with the rotor, or contact Hitachi Koki. While we recommend that 70% ethanol is used for sterilization, no guarantee of sterility or disinfection is expressed or implied.
Page 135: Cabinet
4-3 Cabinet 4-4 Others 4-3 Cabinet Always keep the top deck and the cabinet of the centrifuge clean to prevent dust and other materials from falling into the rotor chamber. Wipe the top deck and the cabinet with a cloth or sponge dampened with a diluted solution of neutral detergent.
Page 136: Troubleshooting
Hitachi Koki may ask you about the treatment for the centrifuge, rotor or the part if the decontamination is checked and judged as insufficient by Hitachi Koki. It is your responsibility to bear the cost of sterilization or decontamination.
Page 137: Alert Indicators
[CE] button. You will then be able to resume your centrifugation. WARNING: Unspecified repairs, remodeling or disassembly of the centrifuge that is not listed in Table 5-1 is strictly prohibited by any person other than a Hitachi Koki authorized sales/service representative.
Page 138 If the rotor life has reached occurs, it indicates that this rotor the primary life, contact reaches the primary life after it is a Hitachi Koki authorized operated for twenty times or 100 sales/service representative to Rotor Life 2 hours or the less.
Page 139: Diagnosed Problems-Requiring Maintenance
There might be abnormality in the heater of the oil diffusion pump. Be sure to contact a Hitachi Koki service representative, if the alert message "E35: DP heater thermistor abnormality" appears. There might be abnormality in the heater of the oil diffusion pump.
Page 140: Installation
Installation or relocation of your centrifuge must be done by the authorized Hitachi Koki service representative. Contact your local dealer or a Hitachi Koki service representative. In order to use the ultracentrifuge fully and safely, follow the installation instructions given below.
Page 141 6. Installation (3) The ultracentrifuge requires a clearance of more than 20 cm at its rear and a floor space of at least 90X90cm (See Fig.6-1). Ensure the surrounding of the instrument has a free circulation of air. Avoid installing the ultracentrifuge near any other heat-generating machine/equipment, which might reduce the ultracentrifuge cooling capacity.
Page 142 6. Installation 3. Fixing the safety cover The ultracentrifuge is shipped with the safety cover removed from its rear. When installing the ultracentrifuge, remove the packing and insert the lower part of the safety cover into the two hooks protruded from the frame and then secure it with the six M4 binding screws (See Fig.6-2). Safety cover (shipping status) Top deck Hook...
Page 143 After moving, be sure to install and level the instrument again. CAUTION: Be sure to remove the rotor from the rotor chamber when centrifuge is moved. After installation and before any test-run, this ultracentrifuge always needs the internal check by a Hitachi Koki authorized sales/service representative.
Page 144: Warranty For The Cp-Nx Series Ultracentrifuge
3. The drive unit shall not be modified, disassembled, or repaired by any party but Hitachi Koki or by a service representative authorized, in writing, by Hitachi Koki.
Page 145: Supply List
8. Supply List 8. Supply List The items below are those supplied with the ultracentrifuge. Item Name Part No. Q'ty Item drawing Remarks Instruction manual S998449 English List of applicable rotors S998453 Instruction manual for rotors,tubes,bottles S203112K and caps Software Simulation CD ass’y S310573A “himac ASSIST”...
Page 146: Appendix
APPENDIX...
Page 147: Decontamination Sheet
Be sure to decontaminate the product according to good laboratory procedures and methods, and fill out this Decontamination Sheet and attach it to the product to be returned to Hitachi Koki for repair. Attention: Hitachi Koki Co., Ltd.
Page 148: Weee Compliance
2002/96/EC (WEEE) Directive. The mark indicates the requirement NOT to dispose the equipment as unsorted municipal waste, but use the return and collection systems available. For further information regarding return, collection, recycle or disposal, please contact a Hitachi Koki authorized sales/service representative.
Page 150 After-Sales Service Periodic inspection of the centrifuge is recommended to assure safe and efficient operation. If the centrifuge fails to function normally, do not attempt to repair it yourself. Contact a Hitachi Koki authorized sales/service representative. 1060, Takeda, Hitachinaka City Ibaraki Pref., 312-8502 Japan...

This manual is also suitable for:

Cp90nx Cp80nx

Hitachi CP100NX Instruction Manual

1 Specifications

2 Description

3 Operation

4 Maintenance

5 Troubleshooting

6 Installation

7 Warranty for the CP-NX Series Ultracentrifuge

8 Supply List

Appendix

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